Measurement Method Laktosa kadar

Preparing to be pondered sample approximately 1 gram and then enter into 500 ml of water, take and put into 2.5 ml test tube bertutup. Dilution factor 500 / 2.5 = 200 times.
Take 2.5 ml and insert the sample into the test tube bertutup. Adding ZnSO4 and 5% Ba (OH) 2 4.5% each 0.2 ml. Sentrifugasi perform at speed 1000 rpm for 5 minutes so that the deposition form. Take Supernatan of 1 ml, and then enter the test tube to a large and bertutup. Adding 2.5 ml of reagent Telles and close the meeting. Soaking tubes in boiling water for 6 minutes, then cool under tap water. Akan form and then read the color orange absorban on 520 nm. Requires a standard solution with the sample and read at 520 nm.
Dilution:
N1 x V1 = N2 x V2 ........................................... .................................( 2)
From reading absorban solution will be the standard price of a, b, r which can be searched with a calculator program that has LINIER / LR.
With the equality Y = a + bx, Y is the sample Absorban
While X =
Kadar Laktosa = (X x dilution factor / sample weight) x 100% ... ... ... ... .... (3)